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Each MS/MS acquisition had an accumulation time of 50 ms and a collision energy (CE) of 30 10 V

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The tissues were homogenized in ice-cold buffer, centrifuged for 30 min at 10,000 g, and the supernatant was kept at -80C for neurotransmitter (ACh, AchE, GABA, 5-HT, and dopamine), oxidative stress markers (MDA), and antioxidant status (GSH, CAT, and T-SOD) determinations