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27 Briefly, the primer pairs forward 5- GCTAGTTAAGCTTGGTACCGAGCTCGGATCCGCCACCATGATGCTTCAAC-3 and reverse 5-CTTTGTAGTCGAAGGGCCCTCTAGACTCGAGGCTCTGCAATGTTC-3 were used to construct the ATF3-WT plasmid and 5-CTTCTGGATCCCTCCCATTCTGAGCCCGGAC-3 and 5-GAATGGGAGGGATCCAGAAGATGAGAGAAACC-3 were used to construct the ATF3 phosphorylation site mutation plasmid (ATF3-Mut)

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117119 The catalytic cycle of GPx is based on a ping-pong mechanism and can be separated in two half-reactions, an oxidative and a reductive phase

AT dysfunction manifests when expansion limits are reached, forcing lipid accumulation in non-ATs and triggering systemic metabolic deterioration (37, 261)

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