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Agarose gels were all 1.5% w/v agarose stained with ethidium bromide (Fischer) and imaged on BioRad GelDoc imager

M.NguyenM

Spread everything on a parchment-lined baking sheet

We found that 19 metabolites were significantly increased by MTX, whereas 12 were decreased in response to the drug treatment ( Figure 1A and Supplementary Table 4 )