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For platelet activation, PRP (5 10 7 platelets/mL) was stimulated for 1 h at room temperature with conditioned PMNL supernatants in the indicated concentrations, in the presence of melagatran (3 mol/L) to prevent secondary thrombin activation

Meanwhile, Mac-1 may be involved in neutrophil cytoskeletal changes and the release of NETs (136)

The accuracy was expressed as relative error (RE) [(mean observed concentration spiked concentration)/(spiked concentration)] 100% and ranged from 1.35 to 2.34% (intraday accuracy) and from 2.84 to 1.63% (interday accuracy)

doi: 10.3390/biomedicines13040986, 83