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This figure highlights the convergence of multiple TCM interventions on the Keap1/Nrf2/ARE axis, GPX4, SLC7A11, ACSL4, and iron transporters, illustrating the multi-target nature of TCM and guiding future combination strategies

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Therefore, early intervention in replenishing mGSH stores by supplying GSH precursor such as N -acetylcysteine (NAC) or targeting upstream mechanisms involved in mitochondrial dysfunction and ROS generation, such as the sustained activation of c-Jun N-terminal kinases (JNK), can result in the protection of APAP-mediated hepatotoxicity

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