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Additionally, although we have established a connection between the anti-acne effects of LP-EVs and their enriched proteins through proteomic analysis and proteinase inhibition assays, future investigations should employ targeted approaches, such as generating mutant bacteria lacking key proteins, exogenously expressing specific candidate proteins, and using small molecule inhibitors to precisely link individual proteins to downstream signaling pathways in the host identified by transcriptomic analysis above and their acne-related biological effects

Among the 15 erinacines (HeA-K and P-S), HeA-I has been found to induce the production of nerve growth factor (NGF) to stimulate neuronal differentiation 15,17,18,19

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