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Description

In a circadian misalignment model (mice kept in the dark for 14 days) in which lower SIRT1 activity was observed, SIRT1 overexpression led to improved insulin sensitivity [31]

The reaction was started by adding 1.0 mL of 200 mM hydrogen peroxide (H 2 O 2 ) in the test cuvette and by adding the same volume of distilled water instead of hydrogen peroxide in the reference cuvette