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K.SongJ.-Y.et al (2018)

The MBOAT1 insert was cloned into pSD292 through a Gibson assembly protocol wherein 100 ng of linearized pSD292 (19.9 fmol) and a 3x molar ratio of insert (59.7 fmol) were combined with the Gibson assembly mix (Cat# E5510S, New England Biolabs) per manufacturer instructions

Most patients are able to return to all activities about 4 to 6 weeks after surgery

Other significant m 6 A readers include the IGF2BP family proteinsIGF2BP1, IGF2BP2, and IGF2BP3which stabilize target mRNAs and enhance their translation [66, 67]