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Pyridinic nitrogen (N-6) and pyrrolic nitrogen (N-5) provided lone pair electrons as Zn 2+ adsorption sites, inducing surface redox reactions

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When these affinity-enhancing mutations were added to full-length rat amylin, all three mutated rat amylin analogs showed enhanced potency for amylin receptor 1/2 activation by 5- to 10-fold compared to endogenous rat amylin and a clinical drug pramlintide (Figure 9A, 9B and Table 3)