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Vacuolar morphology and microscopy To visualize the vacuole, cells in log phase were stained with 8M FM4-64 (Cat # S6689) for 30 min, washed and resuspended in the SD medium, and incubated for an additional 1 hour at 30C (Yamauchi et al., 2015)

Protocols at Perfect B in Doral are individualized, not templated

[1] Cell Line:OVCAR3/4/5/8, SKOV3 and ID8 cells Concentration:0-10 M approximately Incubation Time:48 h Result:Inhibited cell growth with IC 50 values of 1.27, 6.8, 4.33, 3.18, 1.73, 3.75 M

The roGFP2 moiety has two surface exposed cysteines, which undergo disulfide bond formation upon oxidation resulting in an increase in fluorescence excitation intensity at 405 nm along with a relative decrease at 488 nm excitation at a fixed emission of 510 nm (Gutscher et al, 2008)

T cell responses to SARS-CoV-2 spike cross-recognize Omicron