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Additionally, a study identified genetic loci newly associated with accelerated CRC progression in 3-untranslated region of LDHA, which maps to the seed sequence recognised by miR-374a

Given all these in silico inputs, it was decided to choose a tag such as GST (pGEX-4T-1 vector) which could in principle solubilize the polypeptides and refrain it m forming IBs when overexpressed in a host such as E

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