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Sultana R, Mecocci P, Mangialasche F, Cecchetti R, Baglioni M, Butterfield DA

Obtaining diffraction-quality crystals remains the rate-limiting step in solving a novel structure using X-ray crystallography, despite the recent advancements in the areas of molecular cloning, protein production and purification, crystallization screening, data collection using synchrotron radiation, and structure determination programs

These include a low protein diet, low intake of sulfate rich foods, high toxin exposure (which depletes sulfate), low magnesium levels, low molybdenum levels, and issues with the sulfotransferase (SULTs) family of enzymes

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