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Following incubation, cells were rinsed in PBS then incubated with WST-1 reagent (diluted 1:10 into fresh starved media (Roche, UK)) and incubated at 37 C for 4 h in a cell culture incubator according to manufacturers instructions

Specifications: Form: Lyophilised powder Unit Size: 10 mg Appearance: White to off-white solid Storage (unreconstituted): -20 C, dark, dry Reconstitution: Sterile bacteriostatic water Stability (reconstituted): Store at 28 C, short-term Usage Notice: For research use only

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