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The suspension was then added to the SF solution with a volume ratio of 1:2 silk: AuNPs and stirred gently for 10 min to ensure a homogeneous solution

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(29) also demonstrated low serum 25-hydroxyvitamin D levels in patients with alopecia areata, but did not find a clear correlation between serum 25-hydroxyvitamin D levels and the degree of hair loss

The hypoxanthine-guanine phosphoribosyltransferase 1 gene ( HPRT1 ) was used has a housekeeping gene for normalization purposes (Forward: 5 TGACAC TGGCAAAACAATGCA 3, Reverse: 5 GGTCCTTTTCACCAGCAAGCT 3)