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Assessment of glutathione/glutathione disulphide ratio and S -glutathionylated proteins in human blood, solid tissues, and cultured cells

GSH reduces the oxidized catalytic cysteine (Cys47-SOH) of PRDX6, sustaining its peroxidase activity against lipid peroxides, like GPX4, and linking PRDX6 directly to ferroptosis resistance (Fig