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Cells were maintained in DMEM (HCT116, SW480, U2OS), MEM- with 1 ng ml 1 fibroblast growth factor 2 (Peprotech, hMSC) or DMEM with 1% non-essential amino acid (Gibco) and 50 g ml 1 uridine (Sigma-Aldrich) supplementation (143B), with 10% dialysed foetal bovine serum (dFBS), 50 U ml 1 penicillin and streptomycin (ThermoFisher, 15070063) and 1 mM sodium pyruvate (ThermoFisher, 11360070)

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Research suggests GHK-Cu can influence the expression of genes involved in tissue repair, inflammation regulation, antioxidant defense, and extracellular matrix remodelingthe network of proteins and structural components that helps skin remain strong, resilient, and youthful

[19] Outside school, Leigh thrived in the Manchester branch of Labour Zionist youth movement Habonim