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Harvested cells were resuspended in 20 mM HEPES pH 7.0, 500 mM NaCl, 25 mM imidazole (lysis buffer) supplemented with protease inhibitor cocktail and then lysed by a cell disruptor at 25 kpsi (Constant Systems)

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Disruption of skin's natural processes : Your skin has inherent processes for regeneration and equilibrium