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Choosing too small a reconstitution volume

However, transfection efficiency and gene expression are limited compared to viral transduction systems

doi: 10.1016/j.cell.2023.05.003 195 ChenBWangLXieDWangY

These findings suggest that TNBC tumor cells may synergistically utilize the CD39 and PD-1 inhibitory pathways to escape the host immune response, thus leading to poor survival (118)