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In addition, the observed inverse relationship between intracellular sperm levels and average-path velocity (VAP) is important in furthering our understanding of the possible role of free radicals in constraining the actual rate of sperm forward movement within the female reproductive tract, which is vital for successful fertilization

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To validate the pHluorin strategy, we confirmed that exposure of glioma cells expressing GluA2(Q)SEP and PSD95RFP to acidic medium (pH 5.5) quenched the signal, demonstrating that the majority of the AMPAR fluorescent signal at the postsynaptic puncta is from plasma membrane-bound GluA2 (Fig