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Plasma supernatant was collected, divided into aliquots, and frozen at 80 C until further NfL determination, metabolic profiling, or targeted metabolomics

640 Gallic acid treatment can alleviate depression-like behaviors and pain, and the mechanism may be related to inhibiting ferroptosis by regulating the P2X purinoceptor 7 (P2X7)-ROS signaling pathway in spinal microglia

Lipofectamine reagent was used to transfect Phoenix cell cultures with retroviral constructs, which were selected 48 h later with 1 mg/ml puromycin in culture medium

Photothermal therapy of xenografted tumor by carbon nanoparticles-Fe(II) complex

Hand luggage is preferable for temperature stability and reduces the risk of checked-bag delays leaving vials stranded at room temperature