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Invitrogen, E10345) for 30 min, fixed with 4% (v/v) formaldehyde in PBS for 15 min in the dark at RT and then permeabilized with 0.1% (v/v) Triton X-100 for 10 min at RT

However, continuing to maintain this access year after year does not significantly improve care year after year

doi: 10.1016/S0076-6879(05)01028-1

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