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Briefly, standard culture media was supplemented with scheduled addition of adipogenic agents [3-isobutyl-1methyl xanthine (IBMX), Sigma Aldrich

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145 However, they found that even those aged 60 years, taking 2000 mg/day metformin and for 1 year had significantly reduced vitamin B12, indicating that this reduction started early in treatment for all ages and even with low doses

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