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Using immunofluorescence and taking advantage of the HA-tag that marks the transformed cells (Methods), we confirmed the presence of this Clu+/HA+ astrocytic glioma cell population in Notch tumors, while Clu was only seen in HA-negative tumor-associated, untransformed astrocytes in the p53 model (Fig

The treating endocrinologist may decide that some changes are suspicious and order a repeat FNAC or closer follow up

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