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A Laser Doppler Perfusion Imaging analyzer, PeriScan PIM 3 (Perimed, Sweden), was used to measure blood flow of the hindlimb at the 3rd, 7th, 14th, and 21st days post-HLI

doi: 10.3389/fphar.2026.1778496 Received 31 December 2025 Revised 04 February 2026 Accepted 12 February 2026 Published 27 February 2026 Volume 17 - 2026 Edited by Krishna M Boini, University of Houston, United States Reviewed by Wenlong Sun, Shandong University of Technology, China Naciye Yaktubay Dnda, Faculty of Medicine, Cukurova University, Trkiye Updates Copyright 2026 Pi, Yan, Jia, Liu, Tang, Huang, Fang, Shi and Li

International Journal of Pharmaceutics , 532 (1), 6681

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120,000 in 0.1 M Tris buffer with 5% donkey serum and 0.1% Triton X-100) and developed with a Vectastain HRP kit (Vector Labs, Burlingame, CA) and DAB (Metal Enhanced DAB substrate kit, Thermo Scientific, Rockford, Il)