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The invasion assay was done by the same procedure with transwell inserts pre-coated with matrigel (Corning BioCoat, CLS354480, USA), and 5 10 4 tumor cells were added to the upper chamber

The clinical research on GHK-Cu, Palmitoyl Tripeptide-1, Palmitoyl Tetrapeptide-7, and Acetyl Tetrapeptide-3 examined these peptides as focused complexes