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NAD+ and NADH are functionally oppositeNAD+ oxidizes substrates (accepts electrons) while NADH reduces substrates (donates electrons), and the ratio between them governs cellular energy production and sirtuin-mediated longevity pathways

Ferroptosis is defined by the preservation of functional cytoplasmic membranes, increased mitochondrial membrane density, decreased or absent cristae, increased release of oxidized PUFAs, elevated cytoplasmic levels of lipid ROS, and the process can be inhibited by iron chelators (Yang and Stockwell, 2008

As illustrated in Figure 4, the gut microbiota serves as a central hub in diabetic lung injury, where gut-derived metabolites and toxins (e.g., LPS, BCAAs) remotely regulate pulmonary inflammation and immune responses through systemic circulation, ultimately leading to endothelial disruption and oxidative stress

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Laboratory testing methods can detect fragments and metabolites of peptides, emphasizing the reality of a risk of a positive test