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2G-I, Fig S1A)

doi: 10.1016/j.bcp.2018.08.032

C16A/C84A, a double mutant deleting the disulfide from D1

NRF2 deficiency leads to inadequate beta cell adaptation during pregnancy and gestational diabetes

The purified protein was also confirmed using Western Blot with an AcrIIA4 primary antibody and anti-mouse secondary antibody (Cell Signaling Technology # 7076S) (Supplemental Figure 3)