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doi: 10.1016/j.envadv.2022.100203 111 KajlaM.RoyA.SinghI

Their method was direct

While on this regimen, the patients PHQ9 score was an 8 (mild depression)

The appropriate amount of drug solution was added to AuNPs-GSH and pH was maintained in range of 5-8

The following day, after three washes with phosphate buffered saline with tween 20 (PBST), the plate was blocked for 2 hours at 37 C in PBS containing 5% skim milk