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To diagnose hepatic steatosis, it is necessary to demonstrate fat accumulation in more than 5% of hepatocytes on histopathological examination, in the absence of significant alcohol consumption or other causes of liver injury or steatosis, or to detect more than 5.6% fat using proton magnetic resonance spectroscopy (H-MRS) [19]

HPLC and Mass Spectrometry Confirmation High-Performance Liquid Chromatography (HPLC) is the industry standard method for determining peptide purity

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