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Glucose enters the cellular milieu through glucose transporter 1 (Glut-1), followed by conversion to pyruvate, which is mediated by a series of enzymatic steps, including glucose phosphorylation to glucose-6-phosphate (G-6-P), followed by conversion to pyruvate, reducing NAD + to NADH and generating ATP molecules via oxidative phosphorylation (OXPHOS) through the mitochondrial electron transport chain (ETC)

Histological analysis Rat tissues were serially sectioned at 4 m thicknesses and then mounted on slides for further study

Background fluorescence was determined in each individual experiment with cells exposed to Triton (1%) and subtracted from all other values