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Fatty acids (oleic acid, linoleic acid, arachidonic acid, myristic acid, palmitic acid), glycolytic intermediates (glucose, fructose 1,6-bisphosphate) and TCA cycle intermediates (citrate, isocitrate, malate) were increased with GTN treatment

First-strand cDNA was synthesized using the ABScript miRNA First-Strand Synthesis Kit (#RK30170, ABclonal)

Conversely, the relative abundance of Pseudomonadota , Actinomycetota , Cyanobacteriota , Bacteroidota , Thermodesulfobacteriota , Verrucomicrobiota , Kiritimatiellota , Campylobacterota, and Acidobacteriota were significantly reduced ( P 0.05), lower crude fat, and higher crude protein and total collagen ( P 30% were removed, leading to the eventual identification and relative quantification outcomes of the metabolites

Inference of gene regulatory network To elucidate the regulatory mechanisms underlying cancer progression at the single-cell level, we employed the SCENIC method to construct a gene regulatory network

First Consult: Megaloblastic Anemia www.mdconsult.com Rakel: Textbook of Family Medicine, 7th Ed