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Author contributions PM: Resources, Software, Methodology, Writing original draft, Data curation, Project administration, Visualization, Investigation, Validation, Conceptualization, Writing review & editing, Supervision

doi: 10.3389/fphar.2022.839464 86 ChenJLiSGeYKangJLiaoJFDuJFet al

Telomere length testing offers the most direct measurement but carries limitations

While the general principles of N-terminal acetylation and C-terminal amidation are well established in peptide chemistry, their specific effects on the AEDG sequences biological activity, pharmacokinetics in research models, and pathway engagement have not been formally evaluated in peer-reviewed literature (8)

Additionally, subacute oral administration of NSO did not cause any significant toxicity or adverse effects in the animals, indicating that NSO is safe for oral use over a short period