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doi: 10.1038/s41467-019-08294-y 73

For luciferase-based assays cell lines (Raji and MCF7) were modified with plasmid pLenti7.3/V5 TOPO-RedLuc encoding the red luciferase gene and green fluorescent protein, as described previously

An insufficient blood supply to the optic nerve, either due to increased IOP or other vascular risk factors that may reduce ocular blood flow, will induce ischemia and hypoxia processes in the RGCs and their axons, contributing to the development of glaucomatous pathology (Flammer et al., 2002)

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Lipid peroxidation as measured by chromatographic determination of malondialdehyde