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It will also summarize current strategies to enhance MSC adaptability, survival rate, and functional expression, including genetic regulation, biomaterial-assisted transplantation, and microenvironment modulation, to provide a theoretical basis and feasible path for promoting the clinical translation of stem cell therapy in intervertebral disc regeneration

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Unlike a pure cell culture (where individual cells grow in a dish), ex vivo organ culture preserves the complete tissue structure in this case, whole hair follicles with all their cell types intact

Due to inconsistent units of measurement across studies, the standardized mean difference (SMD) was adopted as the common effect size metric, with Hedges g used for small-sample correction (Lifeng Lin and Aloe, 2021)