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184,187,196,197 PCR generates linear dsDNA containing only a single copy of the template and requires thermal cycling, whereas RCA leverages a circular template, exonuclease resistant primers, and a strand displacing polymerase to generate large fragments of mixed ssDNA and dsDNA containing many copies of the template in a single molecule

Conversely, active Rac1-GTP levels were reduced in PFK-deficient cells (Extended Data Fig

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