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Hematoxylin and eosin (H&E) staining After 6 h of ischemia, the liver was then embedded in paraffin after being fixed in 4% paraformaldehyde and dehydrated employing an increasing gradient of ethanol concentration

Vehicle-control MIA mice exhibited no significant change in social novelty, with pre-vehicle scores of 9.1 2.6 and post-vehicle scores of 0.13 4.2, p = 0.23 (Fig 2B)