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What is the difference between primary THA and revision THA coding

RNA was reverse transcribed in a final volume of 20 l containing 1 RT buffer (500 mM each dNTP, 3 mM MgCl2, 75 mM KCl, 50 mM Tris-HCl pH 8.3), 10 units of RNasinTM ribonuclease inhibitor (Promega, Madison, #N2511, WI, USA), 10 mM dithiothreitol, 50 units of Superscript II RNase H- reverse transcriptase (Gibco-BRL, #18064014, Gaithersburg, MD, USA), 1.5 mM random hexamers (Pharmacia, Uppsala, Sweden) and 1 g of total RNA

Specifically, OxS correlates negatively with the integrity of PV neurons and their PNN [15]

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