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The following day, this medium was refreshed, with the expansion medium lacking rho kinase inhibitor but continuing with 5 nM scTS2/16

TABLE 1 Summary of mechanisms leading to tamoxifen resistance The Discovery of LEM4 and the Association Between Cell Cycle Regulators and Resistance to Tamoxifen As a competitive antagonist of estradiol, tamoxifen can bind to estrogen receptors in competition with estradiol and form a stable complex, which inhibits the transcription activity of the estrogen receptor and blocks breast cancer cells in G1 phase to inhibit tumor proliferation

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