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Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template

However, while most of us consume an overabundance of Omega 6's, our Omega 3 intake is woefully lacking

Aloe vera extract in this formula has been processed to remove compounds associated with laxative effects, and the licorice extract is deglycyrrhizinated to reduce concerns associated with glycyrrhizin

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doi: 10.1186/s13287-025-04610-0 9 DouJ.WuY.HuR.LiuJ.ZhangY.ZhenX.et al