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We started oxidative stress experiments by transferring L4 larvae onto the bacterial lawn

Immunoprecipitation of wild-type and mutant forms of GSTP1 After metabolic labelling with -alkynyl-palmitate, cells were washed with cold PBS, harvested, and lysed with cold EDTA-free RIPA buffer [0.1% SDS, 50 mM HEPES, pH 7.4, 150 mM NaCl, 1% Igepal CA-630, 0.5% sodium-deoxycholate, 2 mM MgCl 2 , EDTA-free Complete TM protease inhibitor cocktail] for 15 min at 4C

In accordance with Lipinskis rule of five, we calculated five key physicochemical properties (molecular weight (MW), octanolwater partition coefficient (ALogP), topological polar surface area (TPSA), number of hydrogen bond donors (HBD), and number of hydrogen bond acceptors (HBA)) for the 2070 compounds

Glutathione is an antioxidant naturally found in the body that supports cellular health, oxidative stress defense, detoxification pathways, and overall wellness

Regarding the relationship between vitamin B12 and AMH levels, our study found that the AMH of patients in Low group were significantly lower than those of patients in the High group