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The Ea-domain contains a highly conserved N-glycosylation site (N92) (Fig

Reactions were removed over a time-course, and the digested products were quenched with SDS sample buffer before SDS-PAGE and analysed by western blotting

Most participants were female (236 (77.6%)) and white (283 (93.1%)), with a mean (s.d.) age of 47.3 (11.0) years, body mass index of 38.5 (6.9) kg m 2 and weight of 106.0 (22.0) kg