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The compound was prepared according to the procedure described in Example 1, with the exception that 85.2 mg (0.70 mmol) TRIS was used

Combined analysis of 16S rRNA sequencing and metabolomics reveals the growth-promoting mechanism of compound probiotics in zig-zag eel ( Mastacembelus armatus )

Estimation of proinflammatory cytokines and cortisol levels Serum cortisol, interleukin 1 beta (IL-1), tumor necrosis factor-alpha (TNF-), and interleukin 6 (IL-6) levels were estimated using commercial ELISA kits provided from Cusabio (Wuhan, China) (CSB-E08487f, CSB-E13259Fh, CSB-E13254Fh, CSB-E13258Fh for cortisol, IL-1, TNF-, IL-6, respectively) based on the manufacturer instructions using a microplate reader (Bio-Tek, VT, USA) at absorbance of 450 nm and expressed as ng/mL for cortisol and pg/mL for IL-1, TNF-, and IL-6 (Leng et al

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