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The membranes were blocked with 5% nonfat milk in Tris-buffered saline-Tween 20 (TBST) for 1 h at room temperature before being incubated with primary antibodies against p16, p21 and GAPDH (all from Proteintech, China) overnight at 4 C

Another study showed transfection of cells with miR-133b, a microRNA targeting GSTP1, downregulated certain MMP protein expression and reduced cell migration in nonsmall cell lung cancer (Lin et al