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Cells of 2 L culture were resuspended in buffer 1 (100 mM HEPES-NaOH pH 7.5, 10 mM MgCl 2 ,150 mM NaCl) or in buffer 1 containing 50 mM NaCl, supplemented with 2.5 units/mL Turbo Nuclease (Jena Bioscience) and disrupted by a single passage through a french press at 14,500 psi

fumigatus gliZ were alive at 24 h and the wild-type strain exhibited greater virulence than gliZ (60% (12/20) versus 20% (4/20) mortality, respectively), at 48 h post-inoculation, thereby confirming the utility of the model system for detection of alteration in virulence associated with gliotoxin production

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Vitamin B12 injections are performed by injecting different forms of vitamin B12, including hydroxocobalamin or cyanocobalamin, intramuscularly

Thorel, D