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For determination of intracellular ROS levels upon supplementation of methionine, the bacterial strains were grown to OD of 0.5, washed, and transferred to 7H9 (+ADS) pH 7.2 and pH 5.5, with an additional group of metZ +Methionine

Yellowing of the cake, collapse of the lyophilized structure, or cloudiness after reconstitution are late-stage signs of advanced degradation, not early warning signals