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HA mouse antibody titer ELISA 96-well EIA/RIA plates were coated with recombinant HA (H1N1 A/PR8) protein (1 g/mL/100 L per well) (Sino Biological)

Three additional variables accelerate degradation even when refrigeration is maintained: light exposure, freeze-thaw cycles, and contamination during use

Relative expression from RT-PCR was calculated from the equation 2A-B/2C-D (where A = Cycle Threshold [Ct] number for the gene of interest in the first control sample

Zhang, E., Gao, B., Yang, L., Wu, X

Another feature was that Nrf2 levels declined in ferroptotic macrophages, and ROS levels dropped because Nrf2 overexpression enhanced FSP1