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However, isotope-labeled IS are often expensive or unavailable for certain compounds and using a single isotope-labeled IS for multiple structurally diverse compounds is inappropriate due to differences in retention times, matrix effects, ion suppression, fragmentation patterns, and ionization efficiencies

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Thus, data in support of each hypothesis was derived from different animal models, with mitochondrial oxidative stress being critical for APAP hepatotoxicity in mice on a normal diet, without appreciable LPO generation