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For CYP3A4 inhibition testing, ketoconazole (MilliporeSigma, catalog: PHR1385) was prepared at 2 final concentration in culture medium and added in equal volume to each well of a 384-well plate for 24 h

The gene expression changes the peptide triggers may be more important than continuous peptide presence

In response to injury, this peptide is released to signal a need for healing

Aseptic Technique Requirements All reconstitution procedures must employ strict aseptic technique to prevent microbial contamination: Work in a clean environment, preferably within a laminar flow hood when available Clean rubber stoppers with 70% alcohol swabs before needle insertion Use sterile syringes and needles for all transfers Never touch needle tips or allow contact with non-sterile surfaces Proper Addition Method The physical method of adding solvent significantly impacts reconstitution success