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Description

Injection Process Glutathione drip or shot is injected

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For the immunofluorescence reaction, sections were rinsed in KPBS and incubated for 90 min in AlexaFluor 488 -conjugated secondary antibody (1:500, Jackson Laboratories)

Fusion constructs ROGFP2 WT -PFGRX WT , ROGFP2 WT -PFGRX C88S , and ROGFP2 WT -PFGRX C32S were PCR-amplified with Phusion DNA polymerase using the corresponding p416TEF plasmids as a template 35 and subcloned into the Kpn I and Avr II restriction sites of pET45b in strain XL1-Blue