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Glutathione synthesis is ATP-consuming and depends on the availability of the amino acids cysteine, glutamine and glycine and requires two subsequent reactions, in which -glutamylcysteine synthetase (-ECS or GSH1, EC 6.3.2.2) first catalyzes the formation of -glutamylcysteine (-EC) from cysteine and glutamine and glutathione synthetase (GSHS or GSHS2, EC 6.3.2.3), then catalyzes the formation of glutathione from -EC and glycine

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n-Electrode three-dimensional liquid chromatography with electrochemical detection for determination of neurotransmitters